The impact of radiofrequency exposure on Aedes aegypti (Diptera: Culicidae) development.

INTRODUCTION: Wireless communication connects billions of people worldwide, relying on radiofrequency electromagnetic fields (RF-EMF). Generally, fifth-generation (5G) networks shift RF carriers to higher frequencies. Although radio, cell phones, and television have benefitted humans for decades, higher carrier frequencies can present potential health risks. Insects closely associated with humans (such as mosquitoes) can undergo increased RF absorption and dielectric heating. This process inadvertently impacts the insects' behaviour, morphology, and physiology, which can influence their spread. Therefore, this study examined the impact of RF exposure on Ae. aegypti mosquitoes, which are prevalent in indoor environments with higher RF exposure risk. The morphologies of Ae. aegypti eggs and their developments into Ae. aegypti mosquitoes were investigated. METHODS: A total of 30 eggs were exposed to RF radiation at three frequencies: baseline, 900 MHz, and 18 GHz. Each frequency was tested in triplicate. Several parameters were assessed through daily observations in an insectarium, including hatching responses, development times, larval numbers, and pupation periods until the emergence of adult insects. RESULTS: This study revealed that the hatching rate for the 900 MHz group was the highest (79 ± 10.54%) compared to other exposures (p = 0.87). The adult emergence rate for the 900 MHz group was also the lowest at 33 ± 2.77%. A significant difference between the groups was demonstrated in the statistical analysis (p = 0.03). CONCLUSION: This work highlighted the morphology sensitivity of Ae. aegypti eggs and their developments in the aquatic phase to RF radiation, potentially altering their life cycle.

Multi Beam Dielectric Lens Antenna for 5G Base Station.

In the 5G mobile system, new features such as millimetre wave operation, small cell size and multi beam are requested at base stations. At millimetre wave, the base station antennas become very small in size, which is about 30 cm; thus, dielectric lens antennas that have excellent multi beam radiation pattern performance are suitable candidates. For base station application, the lens antennas with small thickness and small curvature are requested for light weight and ease of installation. In this paper, a new lens shaping method for thin and small lens curvature is proposed. In order to develop the thin lens antenna, comparisons of antenna structures with conventional aperture distribution lens and Abbe's sine lens are made. Moreover, multi beam radiation pattern of three types of lenses are compared. As a result, the thin and small curvature of the proposed lens and an excellent multi beam radiation pattern are ensured.

Multibeam Characteristics of a Negative Refractive Index Shaped Lens.

Narrow beam width, higher gain and multibeam characteristics are demanded in 5G technology. Array antennas that are utilized in the existing mobile base stations have many drawbacks when operating at upper 5G frequency bands. For example, due to the high frequency operation, the antenna elements become smaller and thus, in order to provide higher gain, more antenna elements and arrays are required, which will cause the feeding network design to be more complex. The lens antenna is one of the potential candidates to replace the current structure in mobile base station. Therefore, a negative refractive index shaped lens is proposed to provide high gain and narrow beamwidth using energy conservation and Abbe's sine principle. The aim of this study is to investigate the multibeam characteristics of a negative refractive index shaped lens in mobile base station applications. In this paper, the feed positions for the multibeam are selected on the circle from the center of the lens and the accuracy of the feed position is validated through Electromagnetic (EM) simulation. Based on the analysis performed in this study, a negative refractive index shaped lens with a smaller radius and slender lens than the conventional lens is designed, with the additional capability of performing wide-angle beam scanning.

Increase of Input Resistance of a Normal-Mode Helical Antenna (NMHA) in Human Body Application.

In recent years, the development of healthcare monitoring devices requires high performance and compact in-body sensor antennas. A normal-mode helical antenna (NMHA) is one of the most suitable candidates that meets the criteria, especially with the ability to achieve high efficiency when the antenna structure is in self-resonant mode. It was reported that when the antenna was placed in a human body, the antenna efficiency was decreased due to the increase of its input resistance (Rin). However, the reason for Rin increase was not clarified. In this paper, the increase of Rin is ensured through experiments and the physical reasons are validated through electromagnetic simulations. In the simulation, the Rin is calculated by placing the NMHA inside a human's stomach, skin and fat. The dependency of Rin to conductivity (σ) is significant. Through current distribution calculation, it is verified that the reason of the increase in Rin is due to the decrease of antenna current. The effects of Rin to bandwidth (BW) and electrical field are also numerically clarified. Furthermore, by using the fabricated human body phantom, the measured Rin and bandwidth are also obtained. From the good agreement between the measured and simulated results, the condition of Rin increment is clarified.

Induction of cytotoxicity by Bruguiera gymnorrhiza in human breast carcinoma (MCF-7) cell line via activation of the intrinsic pathway.

Breast cancer is among the frequently occurring cancer worldwide. The foremost underline aim of this study was to determine the growth inhibitory effect along with mechanistic study of a Bruguiera gymnorrhiza extract on MCF-7. The cytotoxicity activity was determined by using the MTS assay. Butanol extract exhibited the maximum cytotoxicity activity against the MCF-7 cells with IC50 of 3.39 µg/mL, followed by diethyl ether and methanol extract (IC50 at 16.22 µg/mL and 37.15 µg/mL, respectively) at 72 h. The DeadEndTM Colorimetric Apoptosis Detection System confirmed the induction of apoptosis (via DNA fragmentation) in MCF-7 cells. Both butanol and diethyl ether extracts of B. gymnorrhiza significantly increase the caspase-3 level. However, the diethyl ether extract induced higher caspase-9 levels compared to caspase-8, suggesting that the intrinsic pathway was the major route in the process of apoptosis. Thin-layer chromatography profiling demonstrated the presence of phenolic, terpene, and alkaloid compounds in crude methanol, diethyl ether, and butanol extracts. The phytochemicals present in the extracts of B. gymnorrhiza might have the potential to be a future therapeutic agent against breast cancer.

Analysis on the Effects of the Human Body on the Performance of Electro-Textile Antennas for Wearable Monitoring and Tracking Application.

Previous works have shown that wearable antennas can operate ideally in free space; however, degradation in performance, specifically in terms of frequency shifts and efficiency was observed when an antenna structure was in close proximity to the human body. These issues have been highlighted many times yet, systematic and numerical analysis on how the dielectric characteristics may affect the technical behavior of the antenna has not been discussed in detail. In this paper, a wearable antenna, developed from a new electro-textile material has been designed, and the step-by-step manufacturing process is presented. Through analysis of the frequency detuning effect, the on-body behavior of the antenna is evaluated by focusing on quantifying the changes of its input impedance and near-field distribution caused by the presence of lossy dielectric material. When the antenna is attached to the top of the body fat phantom, there is an increase of 17% in impedance, followed by 19% for the muscle phantom and 20% for the blood phantom. These phenomena correlate with the electric field intensities (V/m) observed closely at the antenna through various layers of mediums (z-axis) and along antenna edges (y-axis), which have shown significant increments of 29.7% in fat, 35.3% in muscle and 36.1% in blood as compared to free space. This scenario has consequently shown that a significant amount of energy is absorbed in the phantoms instead of radiated to the air which has caused a substantial drop in efficiency and gain. Performance verification is also demonstrated by using a fabricated human muscle phantom, with a dielectric constant of 48, loss tangent of 0.29 and conductivity of 1.22 S/m.